ONCO/Reveal™
Lung and Colon Cancer Genetic Test
What is Lung and Colon Cancer Genetic Test?
It detects numerous gene regions of interest for researchers looking to explore the genetic sequences of both germline and formalin-fixed paraffin-embedded (FFPE) DNA from lung cancer and colon cancer samples. Utilizing SLIMamp® (stem-loop inhibition mediated amplification) allows researchers to amplify regions of interest in a simple, multiplex reaction for subsequent sequencing on a sequencer using a paired-end read length of 150 (2×150).
大多數的肺癌 (~85%) 都歸類為非小細胞肺癌 (NSCLC)。[4] 近一半的 NSCLC 病例與點突變或 EGFR 等其他生物標記有關。這些生物標誌物的流行率因種族而異。亞洲 NSCLC 患者的 EGFR 基因突變比例 (30-40%) 遠高於美國和歐洲的 NSCLC 患者 (10-15%)。[1]
non-small cell lung cancer (NSCLC)
85%
35-40% of patients with CRC found activating mutations in KRAS
10-15%
The proportion of EGFR mutations
30-40%
[1] Ellison, G., Zhu, G., Moulis, A., Dearden, S., Speake, G., & McCormack, R. (2012). EGFR mutation testing in lung cancer: a review of available methods and their use for analysis of tumour tissue and cytology samples. Journal of Clinical Pathology, 66(2), 79–89. https://doi.org/10.1136/jclinpath-2012-201194
大約有 35-40% 的 CRC 患者發現 KRAS 有活化突變。因此，KRAS 基因突變狀態可作為抗 EGFR 靶向治療反應的預測生物標記。目前已有有力證據顯示，西妥昔單抗 (cetuximab) 和帕尼妥單抗 (panitumumab) 對腫瘤帶有野生型 KRAS 基因的 CRC 患者有效。 [2]
Patients with CRC found activating mutations in KRAS
35-40%
[1] Ellison, G., Zhu, G., Moulis, A., Dearden, S., Speake, G., & McCormack, R. (2012). EGFR mutation testing in lung cancer: a review of available methods and their use for analysis of tumour tissue and cytology samples. Journal of Clinical Pathology, 66(2), 79–89. https://doi.org/10.1136/jclinpath-2012-201194
目標
Researchers looking to explore the genetic sequences of both germline and formalin-fixed Paraffin-embedded (FFPE) DNA from lung cancer and colon cancer samples.
Table 1. Gene List
| AKT1 | EGFR | FGFR2 | NOTCH | STK11 |
| ALK | ERBB2 | FGFR3 | NRAS | TP53 |
| BRAF | ERBB4 | KRAS | PIK3CA | |
| CTNNB1 | FBXW7 | MAP2K1 | PTEN | |
| DDR2 | FGFR1 | MET | SMAD4 |
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項目
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Lung and Colon Cancer Genetic Test
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Content
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22 genes related to NSCLC and CRC tumour (please refer to table 1 for details)
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Sequencing Platform
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NGS
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Sequencing Type
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Amplicon based sequencing
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Variants Type
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SNVs, indel
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Sample Type
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FFPE
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TAT
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7 working days**
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流程
STEP 01
售前諮詢
STEP 02
簽署同意書、收集福爾馬林固定石蠟包埋(FFPE) 腫瘤組織塊
STEP 03
將樣本送至實驗室
STEP 04
分析實驗數據
STEP 05
共 7 個工作天完成報告**
**Only applicable to the sample that fulfil the QC requirements.